Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
نویسندگان
چکیده
منابع مشابه
Analysis on the interaction domain of VirG and apyrase by pull-down assay.
VirG is outer membrane protein of Shigella and affects the spread of Shigella. Recently it has been reported that apyrase influences the location of VirG, although the underlying mechanism remains poorly understood. The site of interaction between apyrase and VirG is the focus of our research. First we constructed recombinant plasmid pHIS-phoN2 and pS-(v1-1102, v53-758, v759-1102, v53-319, v320...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2018
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2678